Journal: Drug Design, Development and Therapy

Article Title: Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass

doi: 10.2147/DDDT.S281554

Figure Lengend Snippet: Adenovirus-encoding sh-PBEF reduced the increased phosphorylation of ERK1/2, Akt, and p38MAPK in rat lung tissue. ( A ) Representative blots; ( B ) Phosphorylation of ERK1/2; ( C ) Phosphorylation of AKT; ( D ) Phosphorylation of p38MAPK vs control; Original blots were shown in Supplemental figure 1.*P<0.05; vs LPS, # P<0.05; vs CPB+Blank, @ P<0.05.

Article Snippet: The membrane was incubated with the following antibodies overnight at 4°C: rabbit anti-PBEF (1:2000, 11776-1-AP, Proteintech); rabbit anti-ERK1/2 (1:1000, 16443-1-AP, Proteintech); rabbit anti-p-ERK1/2 (1:500, bs-3016R, Bioss); rabbit anti-p38MAPK (1:1000, bs-0637R, Bioss); rabbit anti-p-p38MAPK (1:1000, bs-5477R, Bioss); rabbit anti-AKT (1:500, ab8805, Abcam); rabbit anti-p-AKT (1:1000, bs-2720R, Bioss); rabbit anti-AQP1 (1:1000, bs-1506R, Bioss); rabbit anti-AQP5 (1:1000, AF5169, Affinity); rabbit anti-ENaC (1:1000, bs-2957R, Bioss); rabbit anti-surfactant protein D (SP) (1:1000, ab220422, Abcam); and mouse monoclonal anti-GAPDH (1:2000, TA-08, ZSBio).

Techniques:

Journal: PLoS ONE

Article Title: Protective Effects of Let-7a and Let-7b on Oxidized Low-Density Lipoprotein Induced Endothelial Cell Injuries

doi: 10.1371/journal.pone.0106540

Figure Lengend Snippet: A: Western-blot assays for NF-κB translocation; B: Percentage of NF-κB (p65) in nucleus; C: IκB protein levels; D: Western-blot assays for p38MAPK, pp38MAPK, pPKB, PKB and IκB protein expression; E: Relative ratio of pp38MAPK/p38MAPK; F: Relative ratio of pPKB/PKB. The values (mean ± SD from three independent experiments) are relative to negative control, which was set as 1. ## P <0.01 vs. NC, * P <0.05; ** P <0.01 vs. oxLDL.

Article Snippet: Secrete-Pair Dual Luminescence Assay Kit was provided by GeneCopoeia Inc.(Rockville, USA); rabbit polyclonal antibody to LOX-1 was provided by Abcam (New Territories, HK, ab60178); mouse anti-p38MAPK monoclonal antibody (sc-81621), mouse anti-p-p38MAPK monoclonal antibody (sc-7973), mouse anti-NF-κB(p65) polyclonal antibody (sc-372), mouse anti-IκBα monoclonal antibody (sc-373893), rabbit polyclonal antibody to eNOS (SC-654), rabbit polyclonal antibody to Histon H3 (sc-8654), second antibody were provided by Santa Cruz Biotechnology Inc.(Texas, USA).

Techniques: Western Blot, Translocation Assay, Expressing, Negative Control